Re up-regulated in normolipidemic subjects in response to FO supplemen…

Re up-regulated in normolipidemic subjects in response to FO supplementation. This bmjopen-2016-011824 finding corresponds with the outcomes of two intervention studies investigating the effect of FO supplementation on whole genome expression in healthy subjects [16,17]. The study of Gorj and co-workers was conducted with ten men aged between 25 and 45 years supplemented with 1.62 g DHA and 0.78 g EPA per day over a period of eight weeks. The design of this study is comparable with our study, except for the sample material used for RNA isolation (fractionated lymphocytes vs. whole blood). Different RNA sources could yield different expression patterns, and cell fractioning itself could alter the gene expression pattern [29]. Nevertheless, both studies provided similar results. Gorj and co-workers discovered 71 up- and 6 down-regulated genes by macroarray analysis. Our normolipidemic FO group showed 627 totalSchmidt et al. Lipids in Health and Disease 2012, 11:105 http://www.lipidworld.com/content/11/1/Page 9 ofTable 3 Selected pathways and genes dominantly regulated in dyslipidemic subjects (FO-D) in response to fish oil supplementation for twelve weeksPathway Lipid metabolism Fatty acid biosynthesis Fatty acid elongation in mitochondria Gene name acetyl-CoA carboxylase beta hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit hydroxyacyl-CoA dehydrogenase Fatty acid metabolism enoyl-CoA delta isomerase 2 enoyl-CoA delta isomerase 1 hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit hydroxyacyl-CoA dehydrogenase acyl-CoA dehydrogenase, short/branched chain PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25116583 aldehyde dehydrogenase 2 family (mitochondrial) Glycerolipid metabolism 1-acylglycerol-3-phosphate O-acyltransferase 6 diacylglycerol kinase, alpha 80 kDa diacylglycerol kinase, eta diacylglycerol kinase, theta 110 kDa aldehyde dehydrogenase 2 family (mitochondrial) monoacylglycerol O-acyltransferase 3 Glycerophospholipid metabolism 1-acylglycerol-3-phosphate O-acyltransferase 6 phospholipase B1 diacylglycerol PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/1435082 kinase, alpha 80 kDa diacylglycerol kinase, eta diacylglycerol kinase, theta 110 kDa phospholipase A2, group IIE phosphatidylserine synthase 1 Arachidonic acid metabolism phospholipase B1 gamma-glutamyltransferase 5 glutathione peroxidase 3 (plasma) glutathione peroxidase 5 phospholipase A2, group IIE Linoleic acid metabolism Alpha-linolenic acid metabolism Immune system Complement and coagulation cascades phospholipase B1 phospholipase A2, group 2-Chloro-3-methoxyaniline IIE phospholipase B1 phospholipase A2, group IIE complement component (3b/4b) receptor 1 coagulation factor III (thromboplastin, tissue factor) fibrinogen gamma chain serpin peptidase inhibitor, clade C (antithrombin), member 1 Cardiovascular diseases Arrhythmogenic right ventricular cardiomyopathy (ARVC) desmocollin 2 calcium channel, voltage-dependent, gamma subunit 5 integrin, alpha 3 transcription factor 7-like 1 Gene symbol ACACB HADHA Entrez_ID 32 3030 Ratio t12 : t0 -4.67 -3.HADH ECI2 ECI1 HADHA3033 10455 1632-2.34 -4.54 6.02 -3.HADH ACADSB ALDH2 AGPAT6 DGKA DGKH DGKQ ALDH2 MOGAT3 AGPAT6 PLB1 DGKA DGKH DGKQ PLA2G2E PTDSS1 PLB1 GGT5 GPX3 GPX5 PLA2G2E PLB1 PLA2G2E PLB1 PLA2G2E CR1 F3 FGG SERPINC1 DSC2 CACNG5 ITGA3 TCF7L3033 36 217 137964 1606 160851 1609 217 1-Methoxy-N-methylpropan-2-amine hydrochloride 346606 137964 151056 1606 160851 1609 30814 9791 151056 2687 2878 2880 30814 151056 30814 151056 30814 1378 2152 2266 462 1824 27091 3675-2.34 -13.69 3.26 2.67 2.47 -3.05 3.4.